Sentence examples for buffer and imaged on from inspiring English sources

Reconstituted chromatin was diluted one hundredfold in 1X PBS, 2 mM MgCl2 buffer and imaged on AP-mica [ 75].

Cells were then treated with indicated substances dissolved in Krebs-Ringer phosphate HEPES buffer (KRPH; 20 mM HEPES, 1 mM CaCl2, 136 mM NaCl, 4.7 mM KCl, 1 mM MgSO4 and 5 mM KH2PO4) or HBSS buffer and imaged on an Olympus IX-81 inverted microscope (Olympus, Tokyo, Japan) in objective-type TIR configuration via an Olympus 60× NA = 1.49 Plan-Apochromat objective.

To assess purity, the purified RNA hairpins were then run on a 15% native polyacrylamide gel for 60 min at 100 V, stained in a 1 10,000 dilution of SYBR Gold Nucleic Acid Gel Stain Life Technologiess, Carlsbad, CA, United States) in 0.5× TBE Running Buffer (Life Technologies) and imaged on a Typhoon FLA 9500 biomolecular imager (GE).

Animals were immobilized in a solution of 20 20 PEG glycerol in phosphate buffered saline (PBS) and imaged on a Nikon Eclipse Ti S fluorescence microscope with a ×20 objective and processed using the software NIS Elements.

Molecular weights were confirmed by reducing SDS-PAGE using NuPAGE 4 12% Bis-Tris Gels in 3- N-morpholino propanesulfonic acid (MOPS) buffer followed by SimplyBlue SafeStain and imaged on the Typhoon Trio imager.

Molecular weights were confirmed by reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using NuPAGE 4 12% Bis-Tris Gels (Thermo Fisher Scientific) in 2- N-morpholino)ethanesulfonic acid (MES) buffer followed by SimplyBlue SafeStain (Thermo Fisher Scientific) and imaged on the Typhoon Trio imager (GE Healthcare).

Southern hybridization was performed at 68° with previously described aqueous buffers (Bass et al. 1994), and the blots were exposed to a PhosphorScreen (GE Healthcare Life Sciences) and imaged on a Typhoon Imaging System (GE Healthcare Life Sciences).

Plates were then rinsed with the washing buffer and imaged using the macro plate reader on a Ti Eclipse Nikon epifluorescence microscope.

Samples were kept hydrated with PBS buffer and imaged immediately after adsorption to glass coverslips.

It discusses the use of OpenCL buffer and image objects.

Following another buffer exchange, the channels were imaged on the microscope.