Sentence examples for buffer and homogenized for from inspiring English sources

Some of coverslips were mixed with 3 ml of PBS buffer and homogenized for 30 sec.

To extract protein from tissues, 0.1 g of tumour tissue was placed in 2.0 ml of cold RIPA buffer and homogenized for 1 min with Polytron-Aggregate (Kinmatica, Luzern, Switzerland).

After incubation at 28°C for 48 h, infected and control cells were lysed by the addition of 600 µl of Buffer RLT (Qiagen) and homogenized for 30s with a rotor-stator homogenizer.

Tissue slices from a single rabbit kidney were combined and fixed in a total of 56 ml RLT buffer (Qiagen, West Sussex, UK) and homogenized for 30 s with an Ultra-Turrax homogeniser with 20 mm probe.

Approximately 30 mg of liver tissue was added to 600 μl of RLT buffer in a RNase-Free tube and homogenized for 30 40 sec using a tissue homogenizer (PowerGen 125, Fisher Scientific).

The insoluble material from the pellet was dissolved in 50 μl of the same buffer containing 5 M guanidinium-HCl (GuHCl), and homogenized for 1 minute followed by vortexing.

Samples of colon were weighed and homogenized for 1 min in phosphate buffer.

Frozen tumors were cut into minute pieces and homogenized for 40 seconds in RNeasy lysis buffer.

The wrist and ankle joints were snap-frozen in liquid nitrogen, ground into a fine powder by mortar and pestle, then lysed with protein lysis buffer (1% Triton X-100, 150 m M NaCl, 10 m M Tris-base, 1 m M EDTA, 1 m M EGTA, pH 7.4, protease inhibitor cocktail; 50 mg specimen per milliliter of lysis buffer), and homogenized on ice for 20 s.

Larvae were exposed for 24 h at 19 °C, then collected into lysis buffer, and homogenized to isolate protein for Western blot analysis (protocol available in the Supporting Information).

Minced tissue samples were suspended in IX CHAPS Lysis buffer and homogenized with a motorized pestle for 20 seconds and incubated at ice temperature for 30 min. Samples were spined at 12000 × g for 20 min at 4°C.