Sentence examples for buffer and heating for from inspiring English sources

Equal amount of protein was denatured by addition of equal volume of 2 × SDS loading buffer and heating for 30 min at 50°C.

The proteins in the eluate supernatants were denatured by addition of SDS sample buffer and heating for 10 min at 75°C.

The middle layer of the supernatant was removed and respun before adding an equal volume of 2× Laemmli buffer and heating for 10 min at 100°C.

Whole cell lysates were prepared by washing the first pellet in PBS followed by centrifugation and resuspension of the pellet directly in 300 μL of SDS-PAGE loading buffer and heating for 10 15 min at 95°C.

Afterwards the proteins were denatured by addition of SDS sample buffer and heating for 10 min at 75°C followed by separation on 12% SDS polyacrylamide-gels (30 µg lysate/lane).

The immune complexes were dissociated by adding 20 μl 3× SDS sample buffer and heating for 5 min at 95°C, and then centrifuged for 1 min at 14,000× g for removing the agarose beads.

After centrifugation at 10,000 rpm for 10 min, the residual cell fragments were mixed with 100 μL of 1% (w/v) SDS-PAGE loading buffer and heated for 10 min in a boiling water bath.

Mouse oocytes were collected in SDS sample buffer and heated for 5 min at 100°C.

P3 was washed once in buffer B, resuspended in Laemmli sample buffer and heated for 5 min. at 95°C.

Nuclei were washed and homogenized directly in loading (Laemli) buffer and heated for 5 minutes at 100°C.

Proteins in the pellets were resuspended in solubilisation buffer and heated for 5 min at 95°C as previously described [31].