Sentence examples for buffer and heating at from inspiring English sources
The reaction was stopped by adding 4X Laemmli buffer and heating at 95 °C for 5 min.
Reactions were terminated by the addition of protein loading buffer and heating at 95°C for 5 min before loading on 10% SDS polyacrylamide gels.
Proteins enriched in SILAC affinity pull-downs were reduced and alkylated, on bead, in 2 mM DTT and 10 mM iodoacetamide respectively before adding sample buffer and heating at 70°C for 10 minutes.
Reactions were incubated at 37°C for 2 hr, followed by quenching with 4 µl 6X SDS-PAGE loading buffer and heating at 95°C for 2 min. 12-µl of reaction (50 pmol of RcsB protein) was resolved in a 12% SDS-PA gel, dried, and phosphor image obtained after 15-min exposure using a storage phosphor screen and a Typhoon Trio Variable Mode Imager and ImageQuant v5.2 software (GE Healthcare).
The reactions were stopped by adding 2 x SDS loading buffer and heating at 95 °C for 5 min.
The reaction was stopped by adding 3 × Laemmli buffer and heating at 95 °C for 4 min.
Pellets were then rapidly thawed, resuspended in 100 μL of lysis buffer and heated at 95 °C for 5 min.
10 μL reactions were mixed with 40 μL sample buffer and heated at 70 °C for 15 min, and 20 μL was run on a 12% polyacrylamide gel.
For solubilization, the extracted membranes were diluted into 30 ml fractions containing 7.2 mg ml−1 of membranes in resuspension buffer and heated at 75 °C for 15 min.
The beads were pelleted by centrifugation at 1000 × g for 30 s, washed five times with 1 ml of binding buffer at 4 °C for 20 min, resuspended in 20 µl SDS sample buffer, and heated at 100 °C for 5 min.
