Sentence examples for buffer and heated for from inspiring English sources

After centrifugation at 10,000 rpm for 10 min, the residual cell fragments were mixed with 100 μL of 1% (w/v) SDS-PAGE loading buffer and heated for 10 min in a boiling water bath.

Mouse oocytes were collected in SDS sample buffer and heated for 5 min at 100°C.

P3 was washed once in buffer B, resuspended in Laemmli sample buffer and heated for 5 min. at 95°C.

Nuclei were washed and homogenized directly in loading (Laemli) buffer and heated for 5 minutes at 100°C.

Proteins in the pellets were resuspended in solubilisation buffer and heated for 5 min at 95°C as previously described [31].

Cells were harvested by centrifugation (10 min, 5200×g), resuspended in 500 µl of 2X Laemmli SDS sample buffer and heated for 5 min at 99°C [67].

The same amount of protein of each sample was suspended in Laemmli denaturing sample buffer, vortexed and heated for 10 min at 70°C.

After centrifugation for 1 min at 13.000 g and room temperature, 30 µl aliquots of PK-digested PMCA-samples were mixed with 30 µl of 2× electrophoresis sample buffer, each, and heated for 10 min at 100°C.

Protein lysates were re-suspended in sample buffer (Thermo Scientific) and heated for 5 minutes at 95°C.

Cells were extracted in Laemmli sample buffer (Bio-Rad) and heated for five minutes at 95°C.

In brief, a minimum of four bacterial colonies was transferred to 200 μl Tris-EDTA buffer, pH 8, and heated for 15 min for 95°C.