Sentence examples for buffer and heated at from inspiring English sources
Samples were diluted in a sample loading buffer and heated at 100°C for 5 min prior to being loaded into electrophoretic gel.
Samples at same concentration in terms of OD = 5 were treated with buffer and heated at 100 °C for 5 min before loading onto the gel.
Serum proteins (2 µl of whole serum or 17 µg of depleted serum) were mixed with lithium dodecyl sulfate sample buffer and heated at 70° C for 10 minutes prior to loading.
The resin was re-suspended in SDS-PAGE loading buffer and heated at 100°C for 5 min. The samples were separated in 10% SDS-PAGE and transferred to PVDF membrane following the standard procedures of Western-blotting.
Ten micrograms of mice lung homogenates were diluted with Laemmli sample buffer and heated at 95°C for 3 min. Proteins were resolved by 12% SDS-polyacrylamide gel electrophoresis and electrotransferred onto a PVDF membrane.
The beads that captured PINK1 complexes were mixed with equal amount of 2× SDS sample buffer and heated at 95°C for 10 min to elute the complex proteins.
Samples were dissolved in LDS sample buffer (Invitrogen) and heated at 70°C for 7 min. Equal amounts of the samples (20 30 µg) were electrophoresed in NuPAGE 4 12% Bis-Tris gel (Invitrogen) and transferred to a 0.45- µm nitrocellulose membrane Bio-Rad Laboratories , Inc). Bio-Rad Laboratories , Inc
The fluorescently labeled microRNAs were mixed with 3× microRNA Hybridization Buffer (Ambion) and heated at 95°C for 3 min before hybridizing with the printed microRNA microarrays for 12 16 h in a 42°C water bath in sealed cassettes.
Cells (5×10) were resuspended in Laemmli buffer (Bio-Rad Laboratories) and heated at 95°C for 10 min.
Homogenates were combined with an equal volume of Laemmli's 2 × gel loading buffer, vortexed, and heated at 95°C for 10 min before gel loading.
Supernatants containing an equal amount of protein extract were supplemented with concentrated 4× LDS sample buffer (Invitrogen) and heated at 95°C for 5 min.
