Sentence examples for buffer and further processed from inspiring English sources

All cellular samples were immediately subjected to the lysis buffer and further processed with RNA extraction according to the RNeasy Mini Kit manual (Qiagen, UK).

ab6276, Abcam plc, Cambridge, UK) diluted 1∶10000 in TBS-T buffer for 1 h at RT, washed three times in TBS-T buffer and further processed as described for SF2/ASF procedure.

Lysate samples were thawed to room temperature and their concentrations equalised to 1 μg/μL (50 μL volume) with RIPA buffer, and further processed for trypsin digestion by sequential reduction of disulphide bonds with TCEP and alkylation with MMTS.

The proposed mechanism benefits from dynamic service level agreement negotiation between a customer and service providers to buffer and further process the potentially blocked high priority connection requests.

Proteins were dissolved in 20 μl of iTRAQ dissolution buffer (Applied Biosystems) and further processed as per the manufacturer's instructions.

Therefore, 100 μL DEPC-treated water was used for resuspension, mixed with 350 μL buffer RLT and further processed according to the manufacturer's protocol.

The recovered PTEN peptide mixtures were then incubated with 20 pmoles Prdx1 (Sigma Aldrich) and 500 μM H2O2 in 20 mM Tris Cl (pH 7.4) buffer at room temperature for 10 min and further processed as described above.

For sample preparation, stool samples were diluted 1 6 (v/v) in the diluent buffer, mixed thoroughly, allowed 5 10 min to settle, and further processed according to the protocol recommended by the manufacturer.

For immunohistochemistry, paraffin sections were deparaffinized, rehydrated in decreasing concentrations of ethanol, treated with 10 mM sodium citrate buffer, pH 6, in the microwave for antigen retrieval, and further processed using the ABC method.

The second halves of the specimens were fixed in 2% glutaraldehyde in PBS and further processed by postfixation in 2% buffered osmium tetroxide and embedded in epoxy resin (Epon 812; Fluka, Buchs, Switzerland) according to standard protocols.

Neurons were then washed, fixed with 1.3% glutaraldehyde in 66 m m sodium cacodylate buffer, incubated with 0.3 mg/ml 3′,3′-diaminobenzidine/0.03% H2O2 up to 30 min and further processed for conventional TEM preparation.