Sentence examples for buffer and frozen at from inspiring English sources

The membrane pellet was resuspended in 100 µl sonication buffer and frozen at −20°C.

DNA was resuspended in 150  μL of elution buffer and frozen at −80°C until use.

Tissue samples for Western blot analysis were placed immediately in the lysis buffer and frozen at -20°C.

After treatments, cells were treated with Qiagen RLT buffer and frozen at −80°C before RNA extraction.

DNA was eluted in 25 μL Tris-EDTA buffer and frozen at −20°C prior to PCR analysis.

P33 cells were detached using Trypsin Catt. #25300, Gibco) and after centrifugation, the cell pellet was lysed in QIAzol buffer and frozen at -80°C.

The proteolysis was started when the protease was added to the protein solution, and the kinetic was stopped by addition of SDS-page loading buffer and freezing at −20°C.

The supernatant was removed by aspiration and cells were lysed in 100 µl Promega reporter lysis buffer (E397A) and frozen at −80°C before luciferase quantitation.

Cells were lysed in lysis buffer (Promega) and frozen at −80 °C for greater than 2 h.

After washing with saline and centrifugation at 350× g (10 min) opsonized zymosan was re-suspended in 10 mL PBS®-glucose buffer, aliquoted and frozen at −20°C.

The supernatants were discarded, and pellets were washed with 50 μl of ethanol and then suspended in 100 μl of sample buffer (29) and frozen at -70°C.