Sentence examples for buffer and freezing at from inspiring English sources

The proteolysis was started when the protease was added to the protein solution, and the kinetic was stopped by addition of SDS-page loading buffer and freezing at −20°C.

The membrane pellet was resuspended in 100 µl sonication buffer and frozen at −20°C.

After treatments, cells were treated with Qiagen RLT buffer and frozen at −80°C before RNA extraction.

DNA was resuspended in 150  μL of elution buffer and frozen at −80°C until use.

Tissue samples for Western blot analysis were placed immediately in the lysis buffer and frozen at -20°C.

Once the F2 progeny had reached L4 the worms were harvested, washed once in M9 buffer and frozen at −80°C.

DNA was eluted in 25 μL Tris-EDTA buffer and frozen at −20°C prior to PCR analysis.

P33 cells were detached using Trypsin Catt. #25300, Gibco) and after centrifugation, the cell pellet was lysed in QIAzol buffer and frozen at -80°C.

Genomic DNA was sequentially precipitated first with isopropanol, then with ethanol and DNA was resuspended in 0.1X TE buffer and frozen at −20°C until further analysis.

Once +80% CD138+ homogeneity is confirmed, the cells are pelleted, placed on RLT (plus 2-mercaptoethanol) buffer and frozen at −80°C.

Cells are transferred into thin walled 0.2 mL PCR tubes containing 2.5  μL of proteinase K/SDS lysis buffer and frozen at −80°C before cell lysis [ 13].