Sentence examples for buffer and fixed for from inspiring English sources

Cells were initially washed in PBS buffer and fixed for 30 min on ice in 4% formaldehyde prepared in CSK buffer.

For FISH, cells were grown to ∼80% confluence on chambered NUNC slides (Invitrogen), washed twice with PBS buffer and fixed for 15 min in 4% formaldehyde in PBS.

Then, cells were washed with acid wash buffer and fixed for imaging.

Third instar Drosophila were dissected in cold cytoskeleton buffer and fixed for 30 min on ice in 4% EM-grade formaldehyde in the same buffer.

Next, the material was washed in the above mentioned phosphatic buffer and fixed for 2 h in 1.0% solution of osmium tetroxide (POCH, Poland) made on the basis of phosphatic buffer.

Flagella were stored in glycerol at −20°C and washed three times in 0.1 M cacodylate buffer and fixed for 10 min with 2.5% glutaraldehyde in the same buffer.

Subsequently, red cells were lysed with ammonium chloride potassium (ACK) buffer, washed, and fixed for flow cytometry (FACSCalibur; BD Biosciences, San Jose, CA, USA).

Ovaries, brains, imaginal discs, and testis were dissected form larvae or adults in Phosphate Buffered Saline and fixed for 15 min with 4% paraformaldehyde in phosphate-buffered saline.

T.cruzi epimastigotes over-expressing AP180-GFP or SNARE 2.1-GFP were washed twice in 0.1 M sodium cacodylate buffer, pH 7.4, and fixed for 1 h on ice with 0.1% glutaraldehyde, 4% paraformaldehyde and 0.1 M sodium cacodylate buffer, pH 7.4.

Culture epimastigotes were collected by centrifugation, washed three times in phosphate buffer (pH 7.2), and fixed for 1 h at room temperature with 0.1% glutaraldehyde + 4% paraformaldehyde in 0.1 M phosphate buffer.

The pellet containing cells was washed with 500 μL of phosphate-buffered saline (PBS; 130 mM sodium chloride and 10 mM sodium phosphate buffer (pH 7.2)) and fixed for 4 h with 4% w/v paraformaldehyde in PBS at 4°C.