Sentence examples for buffer and extraction from inspiring English sources
Parameters affecting the extraction step, such as the amount of sample and EDTA-McIlvaine buffer and extraction solvent volumes, were optimized by means of experimental design.
The aqueous phase was added to the Qiagen extraction buffer and extraction proceeded according to the manufacturer's instructions.
For all samples except A4, DNA was further purified by gel electrophoresis (1% tris-acetate-ethylenediaminetetraacetic acid buffer) and extraction of the appropriate band (Qiagen; exposure to UV was minimized).
All of the wash buffers and extraction buffers included 1× protease inhibitor cocktail (Roche), NaF (1 M), and Na3VO4 (50 μM).
The caryopses were ground in the extraction buffer, and the extraction was centrifuged at 12,000 g for 10 min. The pellet was washed twice then re-suspended in the extraction buffer.
Frozen tissues were ground to a fine powder in liquid nitrogen, transferred to 68°C CTAB extraction buffer, and extracted twice with phenol chloroform (1 1) in equal volumes.
Snap-frozen tissue samples were subjected to mechanical pulverization, followed by disruption of the tissue in lysis buffer and DNA/RNA extraction using AllPrep DNA extraction kits (Qiagen GmbH, Hilden, Germany) according to the manufacturer's recommendation.
Extraction yield was related with four variables, including ratio of solution to solid, extraction power, buffer time, and extraction time.
Subsequently, the cells were washed twice in phosphate-buffered saline (PBS) and treated with lysis buffer and the extraction of transcription factors from the nucleus was performed according to the manufacturer's protocol (Active Motif, Seoul, Korea).
The column was then washed with 5 ml cold GST binding buffer and protein extraction buffer, respectively, and 2 5 ml of total root protein in cold protein extraction buffer were loaded.
Briefly, cell pellets were digested with 0.5 to 2% SDS followed by 0.1 to 0.5 mg ml-1 proteinase K. DNA was purified by incubation with high salt/CTAB buffer and organic extraction.
