Sentence examples for buffer and exposed for from inspiring English sources

After binding, the membrane was washed thrice with binding buffer and exposed for autoradiography.

Using radio-labeled p21-5' DBsitete as probe [25], reaction mixtures were incubated for 30 minutes at RT and then loaded on to 4% native-PAGE containing 0.5 X TBE buffer, subsequently, gel was dried and exposed for autoradiography.

The membrane was washed 2 times with the same solution buffer for more than 30 minutes and exposed for one night.

At the next step, 120 μl target solution of various concentrations in the running buffer solution is injected into the measuring flow cell and exposed for 10 min.

At the next step, 120 μl of target solutions of various concentration in the running buffer solution is injected into the measuring flow cell and exposed for 10 min.

Tissue were incubated in Krebs buffer and exposed to mucolytic solution as described above.

Cells were washed in PBS buffer and exposed to a FITC-conjugated goat anti-mouse IgG secondary antibody (Sigma, diluted 1 100 in PBS) for 1 hour at 37°C.

Briefly, frozen cell pellets were washed twice in wash buffer and exposed to Extraction Buffer I plus protease inhibitor cocktail.

N. attenuata leaves were submerged in infiltration buffer and exposed to a vacuum inside a desiccator.

The membranes were placed into the mixed Detection Buffer and exposed to X-ray film.

Blood plasma from human donors was diluted 1 100 in blocking buffer and exposed to the membranes.