Sentence examples for buffer and equilibrated into from inspiring English sources

Protein-bound beads were washed thoroughly with wash buffer and equilibrated into cleavage buffer (50 mM Tris pH 7.4, 250 mM NaCl, and 1 mM DTT).

After electrophoresis, the gel was soaked in renaturing buffer and equilibrated in developing buffer.

The gel cassette was then opened and the gel was equilibrated into transfer buffer (192 mM glycine, 25 mM Tris, 10% methanol) for 5 10 min. PVDF membrane (Immobilon-FL; EMD-Millpore) was wetted in 100% methanol and equilibrated into transfer buffer prior to transfer-stack assembly.

Reference titrations to account for the heat of dilution of each titrant were performed by titrating into sample cells loaded with the appropriate reaction buffer and equilibrated at the appropriate temperature.

Beads were washed with TAP-lysis buffer and equilibrated with binding buffer (10 mM HEPES pH 7.4, 3 mM MgCl2, 10 mM KCl, 150 mM NaCl).

The plugs were then washed three times with TE buffer and equilibrated with 1× buffer supplied with the restriction enzymes.

Exactly 5 μg of lyz amplicon was diluted into 50 μL of 1XDNaseI reaction buffer (NEB), and equilibrated for 2 minutes at 15°C in a DNA Engine thermocycler (Bio Rad).

All protein samples were concentrated via centrifugal ultrafiltration (1 mM protein for FKBP52 and 0.5 mM protein for FKBP51) and then equilibrated into a pH 6.5 buffer containing 25 mM sodium phosphate, 2 mM DTT and 2 mM TCEP [tris 2-carboxyethyl phosphine] by a series of centrifugal concentration steps.

All protein samples were concentrated via centrifugal ultrafiltration (1000  g for 30 min at 4°C) and then equilibrated into a pH 6.50 buffer containing 25 mM sodium phosphate, 2 mM DTT and 2 mM tris 2-carboxyethyl phosphine by a series of centris 2-carboxyethyl phosphine(1000  g for 30 min at 4°C).

All protein samples were concentrated via centrifugal ultrafiltration and then equilibrated into a pH 6.5 buffer containing 25 mM sodium phosphate (with 2 mM DTT and 2 mM TCEP for the wild-type protein) by a series of centrifugal concentration steps.

Briefly, 900 mg from a frozen tumor was homogenized in 4M guanidine buffer, clarified by centrifugation and equilibrated in 8M urea buffer.