Sentence examples for buffer and diluted for from inspiring English sources

The pellet was dissolved in EB buffer and diluted for sequencing on the Solexa Genome Sequencer.

We successfully extracted DNA for 80 samples, which was resuspended in tris-ethylenediaminetetraacetic acid (TE) buffer and diluted to 20 ng/μl for use in PCR.

In brief, cells were lysed using OpGen lysis buffer and the lysate diluted for direct use.

Filtered protein stock solutions were each prepared in the same buffer solution and then diluted for injection at the desired concentrations.

Plasmids were purified with Endo free Maxi-Prep purification kits (EndoFree Plasmid Maxi Kit, QIAgen) using pyrogen-free material and eluted in pyrogen-free TE buffer in 200 µl/column and then diluted for injection in sterile PBS.

The extracted DNA was suspended in 50 µl TE buffer, and then diluted 1∶20 for use in PCR reactions.

DNA was dissolved in 50 μl TE-buffer for storage and diluted 1 3 in TE-buffer before use.

After coating, the plates were washed once with PBS, 0.1% Tween 20 (washing buffer) and blocked with 300  μl well−1 of PBS, 0.1% Tween 20, 1% BSA (blocking and diluting buffer) for 2 h at room temperature.

DNA obtained was quantified using Nanovue Plus (GE Healthcare, EUA) and diluted in TE buffer for long-term storage.

Sequencing libraries were denatured with sodium hydroxide and diluted in hybridization buffer for loading onto a single lane of an Illumina GA flow cell.

One hundred µL of IgG at a concentration of 0.0205 g/L and diluted in blocking buffer were incubated for 90 min at 18°C.