Sentence examples for buffer and denatured at from inspiring English sources

The N-glycans enzymolysis step was carried out by dissolving 1 mg of ovalbumin in 200 μL glycoprotein denaturing buffer, and denatured at 100 °C for 10 min.

IP pellets were resuspended in SDS-PAGE sample buffer and denatured at 95°C for 10 minutes.

All fractions were combined with equal volume 2× SDS-sample buffer and denatured at 95°C for 5 min. Samples were separated on 4 15% PAGE and blotted with either the anti-AR (N-20; Santa Cruz), the cytoplasmic marker Vinculin (Sigma), or the nuclear marker HDAC (Santa Cruz Biotechnologies).

Neuronal lysates were mixed with sample buffer and denatured at 95°C.

Lysates containing equal amounts of protein were mixed with SDS sample buffer and denatured at 95 °C for 5 min.

The final pellets were resuspended in 40 μl of 2 × Laemmli buffer and denatured at 100°C for 5 min.

Immediately prior to hybridization, labelled aRNA samples were fragmented (Ambion Fragmentation buffer, Ambion) and denatured at 95°C for 10 minutes.

The secreted protein fraction was dissolved in 6x SDS-PAGE buffer containing DTT, and denatured at 95°C for 3 minutes.

Briefly, mRNA (10 μg) was incubated in labeling buffer (25 μL) and denatured at 95°C for 5 min and cooled on ice.

For western blot analysis, 20 μg of total protein was mixed in 2× Laemmli buffer (Sigma-Aldrich) and denatured at 95°C.

Apoplastic fluids collected from two plants for each sample were pooled, dissolved in 6× SDS-PAGE buffer containing DTT, and denatured at 95°C for 3 minutes.