Sentence examples for buffer and cultured in from inspiring English sources

Before embarking on a ChIP-seq experiment, differentiated 3T3-L1 adipocytes were washed with 1× phosphate-buffered saline (1× PBS buffer) and cultured in DMEM supplemented with 5% stripped FBS (J.R. Scientific) for 24 hours.

The swabbed samples were serially diluted in buffered peptone water and cultured in the same regime as plastic jerry can containers surface swabs.

One day before performing the assay, liver spheroids and monolayer cultures were washed three times with phosphate buffered saline (PBS; Corning) and cultured in assay medium, consisting of Williams E (PAN-Biotech) with 5.5 mM glucose and 0.1% bovine serum albumin (Serva, Heidelberg, Germany), overnight.

Larvae were washed in phosphate buffered saline (Boston BioProducts) and cultured in 24 well plates (Becton Dickinson).

A mock control was electroporated using transfection buffer without the disruption vector and cultured in regular medium.

Coverslips were mounted in a heated stage circular chamber and cultured in buffer containing 10 mM HEPES, 147 mM NaCl, 2 mM KCl, 2 mM CaCl2, 1 mM MgCl2, 13 mM d-glutamine.

As reported previously, the liposomes were prepared and cultured in HEPES buffer (pH 7.4, 10 mM HEPES, 150 mM NaCl) such that their molar concentration was adjusted to 100 μM [ 20].

Bone chips were rinsed three times in phosphate buffered saline (PBS), broken into small pieces and cultured in alpha-MEM supplemented with 10% FBS.

After injection, embryos were take out from the injection troughs and cultured in 0.3× Danieau's buffer at 28.5°C until being examined.

After injection, embryos were recovered from the injection troughs and cultured in 0.3× Danieau's buffer at 28.5°C until being examined.

Cells were rinsed three times using PBS and cultured in secondary fluorescent-conjugated antibodies (Jackson ImmunoResearch) at 1 200 dilution in blocking buffer.