Sentence examples for buffer and cryosectioned into from inspiring English sources

Hearts were extracted, fixed in 4% PFA (in Phosphate buffer) and cryosectioned into 14 µm thin sections.

Organotypic slice cultures were fixed and cryosectioned into successive 20 µm slices for immunostaining.

Brains were post-fixed overnight in 4% PFA, cryopreserved in 30% sucrose and cryosectioned into 40 µm sagittal sections.

The specimens were then dehydrated in 30% sucrose, embedded in OCT, and cryosectioned into 10- μm sections.

Samples were embedded in OCT (Tissue-Tek) and cryosectioned into 6-μm-thick sections or embedded in paraffin and cut into 5-μm-thick sections.

Cell-laden beads were harvested on days 0 and 35, rinsed with 0.9% saline, fixed in 4% paraformaldehyde at room temperature for 4 h, embedded in OCT tissue freezing medium (Tissue-Tek), and cryosectioned into 10  μm thick sections.

Whole brains were removed, embedded in paraffin, and cryosectioned parasagittally into 10 μm serial sections.

Frozen tumor and lung tissue samples were cryosectioned into 10-20 µm slices.

The frozen tissues embedded in OCT were cryosectioned into 5  μm thickness and blocked in phosphate-buffered saline (PBS) with 2.5% goat serum for 30 min.

The tumor samples were frozen in OCT® medium, cryosectioned into 100 μm slices, and subsequently stained with anti-CD31 antibody.

After freezing with dry ice, biopsies were cryosectioned into 8 μm slices and mounted on a slide.