Sentence examples for buffer and concentrated for from inspiring English sources

The core RNAP was then dialyzed into carbonate buffer and concentrated for labeling as described below.

Protein of >95% purity (by SDS/PAGE analysis) was exchanged into 50 mM Tris-HCl, 100 mM NaCl (pH 7.5) buffer and concentrated for activity analyses.

The eluant was collected and concentrated for further purification using gel filtration chromatography (Superdex-200 column, GE Healthcare) in buffer containing 20 mmol/L HEPES pH 7.5, 200 mmol/L NaCl, 2 mmol/L DTT on the FPLC system (GE Healthcare Life Sciences).

The protein solution was then dialysed against the NMR buffer and concentrated as for the protein purified from the soluble fraction.

The protein and RNA solutions were repeatedly exchanged into ITC buffer and concentrated using centrifugal concentrators.

For crystallization the protein was dialyzed against 20 mM Tris-HCl, 200 mM NaCl at pH 8.0 buffer and concentrated to 28.4 mg ml−1.

For purification, 50 μg BAC DNA of clone#11 BAC-Col10a1-LacZ-neo) were dissolved in TE buffer and concentrated to 500 μl by vacuum centrifugation.

The reaction products were dissolved in 3 μl loading buffer and concentrated with a vacuum desiccator.

The peak fractions were concentrated and further purified on HPLC (Millipore) Superose 12 FPLC column by elution with native buffer and concentrated to 2.5 mg/mL.

The conditioned media was buffer exchanged and concentrated before being processed for analysis by LC-MS/MS.

For each detergent, ∼20 μL of eluted protein was diluted to 500 μL with exchange buffer (see Protein Expression and Batch Purification section for buffer contents) and concentrated to ∼100 μL using a 3.5 kDa spin column (Millipore , Inc.