Sentence examples for buffer and collected as from inspiring English sources

The sporozoites were eluted in 10 ml of equilibration buffer and collected as 500 µl fractions.

The pellet was solubilized in 100 μL of SDS-sample buffer and collected as detergent-insoluble pellet (ppt).

The mixture was centrifuged at 10 000  g for 10 min, after which the pellet was washed with isolation buffer again and collected as purified mitochondria.

Cultures were harvested by centrifugation at 1600 g for 10 min, resuspended in two volumes of homogenization buffer and TM collected as described (Borner et al, 2005).

An additional 3.5 ml of PBS was added to the column, and the displaced buffer was collected as the elution fraction.

After the treatment, the buffer was collected as the extracellular fraction.

The remaining infiltration buffer was collected as "supernatant".

The cells were grown for a further 2 h at 30 °C, harvested by centrifugation (6000 g, 10 min, 4 °C), resuspended in PBS buffer and collected by centrifugation as before.

Following incubation, 50 μl of preheated 95°C hot SDS gel loading buffer was added and elute was collected as immunoprecipitate.

Finally, the protein suspensions were digested with 2 μg trypsin (Promega) in 40 μl DS buffer overnight at 37 °C, and the resulting peptides were collected as a filtrate.

From each site, the tissue was collected as fresh frozen and also in RNA-stabilizing buffer.