Sentence examples for buffer and cloned into from inspiring English sources

PCR fragments were analyzed by electrophoresis in a 1% agarose gel (1x TAE buffer) and cloned into pJET1.2/blunt vector using the CloneJET PCR Cloning Kit (Fermentas).

The promoter region (2000 bp) of human Klf4 promoter was amplified using PrimerSTAR HS DNA Polymerase with GC buffer (TaKaRa BIO Inc ,DR010GC) and cloned into pGL3-vector with double-digestion of KpnI and HindIII.

Genomic DNA containing SAK1 was amplified using primers 5′-CAGGACCGGGCACTGAGTGAAGGTTA-3′ and 5′-ATGATGCACTGTGGGACACGCTGAGT-3′ using PrimeStar HS with GC buffer (Takara/Clontech, Palo Alto, CA) and cloned into pGEM-Teasy after adding an adenine.

DNA fragments were separated in a 2% 1 × TAE buffered agarose gel, extracted using NucleoTrap (Macherey-Nagel) and cloned into pGEM-T Easy vector (Promega).

The Sepharose beads were washed four times in ice-cold lysis buffer, and the bound proteins were eluted with 1×SDS-PAGE sample buffer at 90°C for 10 min. Various regions of AGS3 were PCR amplified and cloned into pGEX4T2 (GE Healthcare).

Both ORFs were cloned with Invitrogen Topo TA Cloning kit, digested using EcoR1 and cloned into PCS2+.

The resulting PCR product was purified and cloned into the pCRII TOPO cloning vector (Invitrogen).

BIG1 fragments were PCR amplified and cloned into pGEX4T2 (Pharmacia).

PCR products were purified and cloned into pBluescript-KS-M13+.

The PCR product was purified and cloned into pPCRScript Amp, and resulting clones were sequenced.

PCR products were electrophoresed in 2% agarose gels using 1x TAE buffer (40 mM TRIS, 20 mM sodium acetate, and 1 mM EDTA adjusted to pH 7.2 with glacial acetic acid) with ethidium bromide and purified (Gel-Out Kit, A&A Biotechnology, Poland) and cloned into the pJet 1.2 vector (CloneJET PCR Cloning Kit, Thermo Scientific, USA) in accordance with the manufacturers protocols.