Sentence examples for buffer and cells were from inspiring English sources

Erythrocytes were lysed using NH4Cl buffer, and cells were resuspended in media.

Following incubation, the cells were washed three times with FACS buffer and cells were fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Inc., Warrington, PA).

Aliquots of cells (1×107) were incubated with DAPI for 20 minutes, washed and resuspended in FACS buffer, and cells were analyzed on a BD LSR II flow cytometer.

Following incubation, the cells were washed three times with FACS buffer and cells were fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Inc., Warrington, PA) diluted in FACS buffer (fixation buffer).

Primary antibodies were diluted in blocking buffer and cells were incubated for 1 hr at room temperature.

The reaction was stopped by washing cells twice with 1 mL of ice-cold wash buffer, and cells were lysed in 1 mL of 0.2 M NaOH.

Staining buffer was removed and cells were washed with buffer.

The buffer was removed and cells were resuspended in 250 μl of cold electroporation buffer and electroporated at 250 V and 960 mF with 5 μg of DNA.

Subsequently, loading buffer was removed, and cells were returned to pre-warmed media and incubated for 30 min before the final reading.

After incubation, 200 µl of buffer was added and cells were centrifuged.

After incubation, 200 µl buffer was added and cells were centrifuged.