Sentence examples for buffer and boiling at from inspiring English sources

The reaction was stopped by SDS sample buffer and boiling at 95°C for 3 min.

The reaction was stopped by adding 2× loading buffer and boiling at 100 °C for 15 min.

Reactions were stopped by the addition of SDS PAGE sample buffer and boiling at 95 °C for 5 min. Samples were then analysed by western blotting.

The resins were washed three times with lysis/binding/washing buffer and the samples were eluted by adding 50 μl of 2X SDS sample buffer and boiling at 95°C for 5 minutes.

Reactions were incubated at 37 °C for 20 min and were terminated with the addition of 15 μl 5 × SDS PAGE sample buffer and boiling at 95 °C for 5 min. In vitro kinase assays using recombinant CDK2/cyclinA at 40 ng/μl with 0.2 μg/μl GST-Eg5 were started by adding 25 μM ATP.

We flash froze the embryos followed by grinding with a glass pipette in SDS loading buffer and boiling at 95°C for 5 min. We probed the membranes with anti-HP1E and anti-beta actin (Abcam, Cambridge, UK, both at 1 5000).

The supernatant and sediment were respectively disposed with SDS-PAGE loading buffer and boiled at 100 °C for 5 min.

The lysates mixed with a certain amount of 5× loading buffer and boiled at 100 °C for 10 min.

Beads were washed 3 times and the immunoprecipitated proteins were eluted with 2× Laemmli buffer and boiled at 100°C.

Samples were then combined with 2X loading buffer and boiled at 80°C for 10 min prior to loading.

Beads were washed 3 times with lysis buffer and the immunoprecipitated proteins were denatured with 2x Laemmli buffer and boiled at 100°C.