Sentence examples for buffer and boiled for from inspiring English sources

Briefly, cells were washed twice in phosphate-buffered saline (PBS) and cell pellets were lysed in electrophoresis buffer and boiled for 10 min.

All reactions were stopped by adding 5 × SDS loading buffer and boiled for 10 min at 95 °C for WB analysis21.

The total of 30 μg/mL of cell lysates were resuspended in Laemmli loading loading buffer, and boiled for 10 min at 95 °C.

The precipitates were mixed with 5× western blot loading buffer and boiled for 5 min.

1011 phage particles were mixed in Laemmli buffer and boiled for 5 min.

Whole cell lysates were suspended in lamaelli buffer and boiled for 15 minutes, followed by centrifugation.

Powder was transferred to boiling SDS-sample buffer and boiled for 5 minutes.

Precipitated proteins were eluted with 2× SDS-PAGE sample buffer and boiled for 10 minutes.

Immunoprecipitates were washed thoroughly prior to resuspension in 2x Laemmli sample buffer and boiled for 3 minutes.

Two volume of protein samples were mixed with one volume of 3× Laemmli buffer and boiled for five minutes.

The total cell lysates were mixed with 5× Laemmli buffer and boiled for 5 min at 100°C.