Sentence examples for buffer and boiled at from inspiring English sources

The lysates mixed with a certain amount of 5× loading buffer and boiled at 100 °C for 10 min.

The supernatant and sediment were respectively disposed with SDS-PAGE loading buffer and boiled at 100 °C for 5 min.

Samples were then combined with 2X loading buffer and boiled at 80°C for 10 min prior to loading.

Beads were washed 3 times and the immunoprecipitated proteins were eluted with 2× Laemmli buffer and boiled at 100°C.

Beads were washed 3 times with lysis buffer and the immunoprecipitated proteins were denatured with 2x Laemmli buffer and boiled at 100°C.

The homogenates were mixed with sample buffer and boiled at 95°C for 5 min. Ten microgram of protein was resolved on 15% SDS-PAGE, and transferred to polyvinylidene difluoride membranes (Millipore).

In order to isolate the proteins bound to the biotynilated miRNAs, washed beads were resuspended in SDS-buffer and boiled at 100°C.

The reaction was stopped by SDS sample buffer and boiling at 95°C for 3 min.

The reaction was stopped by adding 2× loading buffer and boiling at 100 °C for 15 min.

Reactions were stopped by the addition of SDS PAGE sample buffer and boiling at 95 °C for 5 min. Samples were then analysed by western blotting.

The resins were washed three times with lysis/binding/washing buffer and the samples were eluted by adding 50 μl of 2X SDS sample buffer and boiling at 95°C for 5 minutes.