Sentence examples for buffer and blots were from inspiring English sources

Antibodies were diluted in the respective blocking buffer and blots were incubated O/N at 4°C.

Antibody dilution was prepared in blocking buffer and blots were incubated with monoclonal antibody overnight at 4°C.

After extensive washing, the immunoprecipitates were heated in SDS sample buffer, resolved by SDS/PAGE and blots were probed with the antibodies indicated.

Excess radioactive probe was washed out with 2x SSC, 0.1% SDS buffer, and the blots were exposed for one week to phosphorimaging screen (Fujifilm) and scanned with Fujifilm FLA5100 reader (Fujifilm Co., Ltd., Tokyo, Japan).

The supernatant was mixed with 5X loading buffer and the blots were run using the NuPage Novex 4 12% gradient Bis-Tris gels (Invitrogen) with MOPS running buffer (Invitrogen).

Spheres were collected, resuspended in 1x RIPA lysis buffer and western blots were performed according to standard protocols.

Reactions were stopped by the addition of SDS sample buffer and western blots were performed after electrophoresis of samples on 4 20% Tris-HCl gels.

Proteins bound to beads were eluted in SDS sample buffer, and Western blots were performed.

After hybridization, the membrane was washed with low and high stringency buffers and the blots were exposed to Biomax-MS film (Eastman KODAK, Rochester, NY).

After UV cross-linking and prehybridization in UltraHyb buffer (Applied Biosystems/Ambion), blots were hybridized in the same solution with a P-labeled antisense RNA probe corresponding to the PPV P1 or NIb coding sequence, previously hydrolyzed with carbonate buffer to an average length of approximately 50 nt.

After washes in blocking buffer, the blots were incubated simultaneously in 2nd Abs linked to Cy3 and Cy5.