Sentence examples for buffer and assayed for from inspiring English sources

Membranes (equivalent to 350 µg of proteins) were recovered by ultracentrifugation of the total extract at 100, 000×g for 20 min at 4°C, washed twice with potassium phosphate buffer and assayed for desaturase activity.

The corresponding precipitates were recovered, dissolved individually in fresh buffer and assayed for both total protein content and cellulase activity.

After transfection, the cells were washed with PBS, lysed with 1× passive lysis buffer and assayed for luciferase activity using the Dual-Luciferase Reporter Assay System (Promega, USA).

Samples were subsequently homogenized in buffer and assayed for MDA content using the thiobarbituric acid (TBA) reaction, as described by Uchiyama and Mihara [ 18]. with some minor modifications.

After 48 h, the cells were lysed in passive lysis buffer and assayed for both firefly and renilla luciferase activity using the Dual-GLO® Luciferase Assay System (Promega).

After 36 hours, the cells were washed with PBS, lysed with 100 μl of passive lysis buffer, and assayed for luciferase activity as relative light units using the Dual Luciferase assay system (Promega).

After additional 24 h, the cells were harvested in phosphate-buffered saline, lysed in luciferase lysis buffer (Promega), and assayed for luciferase and beta-galactosidase activities using the Promega luciferase assay system (Promega).

24 hours post-transfection, cells were harvested in 1× PBS, lysed in Passive lysis buffer (Promega) and assayed for luciferase and β-galactosidase activities using a Fluostar galaxy plate reader (BMG Labtechnologies).

Transfected cells were lysed in reporter lysis buffer (Promega) and assayed for luciferase and renilla activities using the Dual-Luciferase Assay System (Promega).

To determine the luciferase activity, cells or tissues were lysed in Passive Lysis Buffer (Promega) and assayed for firefly (F-luc) and Renilla (R-Luc) luciferase according to the Promega Luciferase assay kit instructions in a Turner Designs (Sunnyvale, CA) TD-20/20 TD-20/20rMingen Morolight 3010 luminometer.

Pituitaries were homogenised in phosphate-buffer saline and assayed for anterior pituitary hormones using National Hormone and Pituitary Program reagents kindly provided by A.L. Parlow, as described in [25].