Sentence examples for buffer and applied on from inspiring English sources

The pellet was resuspended in 500 μl H-S buffer and applied on the top of a HEPES-buffered sucrose step density gradient (0.3 ml of 2.3 M, 1.7 ml of 1.7 M, and 1.5 ml of 1 M).

The resulting supernatants were combined and centrifuged at 17500 g for 15 min. The pellet was resuspended in HOMO-A buffer and applied on the top of a step gradient with interfaces of 0.65 M, 0.85 M, 1 M, 1.2 M sucrose in HOMO buffer.

Briefly, a capturing antibody (goat anti-human IgM/IgG) was prepared in a carbonate/bicarbonate buffer and applied on a 96-well plate in a concentration of 100 ng/100 μL/well.

SDS-PAGE: 3μl of each sample were diluted in ONYX lysis buffer, mixed 1 3 with laemmli loading buffer and applied on a 14-20% precast gradient polyacrylamide gel.

Purified complexes were washed five times with the lysis buffer and applied on SDS-PAGE followed by silver staining according to the manufacturer's instructions (Invitrogen) or Western blotting procedure.

After incubations, specimens were heated at 95°C for 5 min, diluted with 10 volumes of buffer A, and applied on 1 ml DEAESepharose gel equilibrated in the same buffer.

Serum samples were thawed on ice and 1 ul serum was diluted in 50 ul serum binding buffer and applied to spots and left on shaker (250 rpm) at room temperature for 40 minutes.

B. asper crude venom (300 mg) was dissolved in 1.5 mL of 0.05 M ammonium bicarbonate buffer, pH 8.1 and applied on a CM-Sepharose column (1.5 × 15 cm) according to Soares et al. [ 17].

Protein extracts (200  μg from each sample) were precipitated following the methanol/chloroform procedure [ 15], resuspended in 100  μL Laemmli sample buffer, and applied onto 1.2 cm wide wells on a 12% SDS-PAGE.

Cells were then washed twice with MACS buffer by centrifugation, resuspended in 1.5 ml of MACS buffer and applied to a presoaked MiniMACS column (130-042-201; Miltenyi Bionec) on a magnetic backing.

Secondary antibodies were diluted in blocking buffer and applied to membranes for 1 h at room temperature.