Sentence examples for buffer and applied for from inspiring English sources

Biotinylated goat anti-rabbit (VectorLabs, Burlingame, CA) was diluted 1∶400 in the blocking buffer and applied for 1 h at 25°C.

Reactions were stopped by addition of SDS buffer and applied for gel electrophoresis.

Primary antibodies were diluted in blocking buffer and applied for 1 hr at room temperature or overnight at 4°C.

After three washes in PBS, secondary antibodies conjugated to Alexa fluorophores (Life Technologies) were diluted at 1 1000 in blocking buffer and applied for 1 hr at room temperature.

DAB working solution made up of 1 20 DAB chromogen in DAB substrate buffer was prepared and applied for 5 min.

Secondary antibodies of goat anti-mouse Alexa-conjugated, goanti-rabbitbit Alexa-conjugated (Molecular Probes) were diluted at 1∶500 in blocking buffer and applied to cells for 1 hour at room temperature.

Briefly, serum samples were diluted 1/25 in PBS-Tween buffer and applied to the wells for 1 h at 37 °C, before washing three times with PBS-Tween and applying anti-bovine IgG conjugate (provided in the kit) for 1 h.

EpiDerm™ cultures were homogenized in lysis buffer and applied to a glass fiber filter spin column for total RNA isolation (RNAqueous®, Ambion, Inc., Austin, TX).

Vimentin was assembled in phosphate assembly buffer at 0.2 mg/ml for about 25 min. Then, the sample was diluted 40 to 60 fold in the same buffer and applied to the surface.

Zymographic detection of ATPase activity in total saliva was performed by separating the proteins at 4°C in native conditions on an 8% polyacrylamide gel using TAE (40 mM Tris, 5 mM sodium acetate, 1 mM EDTA) as running buffer and applying a voltage of 100V for 3 hours.

Either rat anti-mouse CD31 or isotypic antibodies 1∶100 in 10× diluted blocking buffer were applied for 1.5 h and then sections were washed with PBS.