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Exact(32)
Finally, beads were boiled with loading buffer and analyzed on a 12% SDS-PAGE gel.
Cells were lysed in RIPA buffer and analyzed on immunoblots as described before [26].
The samples were diluted 1 1 in reducing sample buffer and analyzed on SDS-PAGE.
Cells were resuspended in 0.5 ml buffer and analyzed on a BD FACSCalibur flow cytometer.
The cells were washed and resuspended in MACS buffer and analyzed on a FACSCalibur flow cytometer as described above.
After washes, the cells were suspended in FACS buffer and analyzed on a FACSCalibur (BD Bioscience, San Jose, CA).
Similar(28)
For the flow cytometry, cells were suspended in Propidium Iodide DNA staining buffer [87], and analyzed on a Becton Dickinson FACScan Analytic Flow Cytometer at the UCLA Flow Cytometry Core Facility.
Cells were washed, resuspended in PI/RNase staining buffer (BD), and analyzed on a BD Biosciences FACSVerse flow cytometer.
After three more washes in PBS, cell pellet was re-suspended in 10% buffered formalin and analyzed on a Coulter EPICS XL AB6064 flow cytometer (Beckman Coulter, Fullerton, CA).
Cells were washed twice, resuspended in 1 mL of FACS buffer and analyzed for FLOW on a Beckman-Coulter FC500.
The stained cells were centrifuged, washed twice with the wash buffer provided in the kit, resuspended in 500 μl of the same buffer, and analyzed for fluorescence on a FACSCalibur flow cytometer using CellQuest software.
More suggestions(14)
buffer and visualized on
buffer and kept on
buffer and placed on
buffer and separated on
buffer and sonicated on
buffer and homogenized on
buffer and depended on
buffer and fractionated on
buffer and resolved on
buffer and chilled on
buffer and lysed on
buffer and applied on
buffer and mounted on
buffer and layered on
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