Sentence examples for buffer and analyzed for from inspiring English sources

Protein fractions were dialyzed against unlinkase buffer and analyzed for unlinkase activity.

Fractions were collected, dialyzed into unlinkase buffer, and analyzed for unlinkase activity.

Protein fractions were dialyzed into unlinkase buffer and analyzed for unlinkase activity; fractions containing peak activity were then used in further assays.

Cells were harvested in SDS sample buffer and analyzed for IB with indicated antibodies.

Cells were washed twice, resuspended in 1 mL of FACS buffer and analyzed for FLOW on a Beckman-Coulter FC500.

The stained cells were centrifuged, washed twice with the wash buffer provided in the kit, resuspended in 500 μl of the same buffer, and analyzed for fluorescence on a FACSCalibur flow cytometer using CellQuest software.

Cells were washed once with PBS, lysed using Passive Lysis Buffer (Promega), and analyzed for luciferase activity using the Dual-Luciferase Reporter Assay System (Promega), according to the manufacturer's instructions on a FB12 Sirius luminometer (Berthold Detection Systems, Oak Ridge, TN).

Briefly, tissues were homogenized in a cytoskeletal extraction buffer (50 mM Tris-HCl, pH 6.8; 200 mM NaCl, 1% Triton-X-100, 20% glycerol, and 1 mM EDTA), centrifuged, and the pellets containing the cytoskeletal fractions were suspended in the same buffer, sonicated, and analyzed for protein content.

Input/output measurements were recorded in Mg2+-free buffer in triplicate and analyzed for population spikes.

Cells were fixed with 70% EtOH, washed with PBS, stained using PI/RNase staining buffer (BD Biosciences) and analyzed for cell cycle with a flow cytometer.

Subsequently, cells were centrifuged, supernatant discarded, 200 µl FACS Buffer (BD Biosciences) added, and analyzed for surface marker expression by flow cytometric analysis.