Sentence examples for buffer and analyzed as from inspiring English sources

After labeling, cells were washed, lysed in native lysis buffer, and analyzed as above.

Samples were then dissolved in radioimmunoassay buffer and analyzed as described elsewhere [ 53].

The lumen was first flushed with EDTA PBS buffer, and then, tissue was collected and homogenized in PBS buffer and analyzed as unit per milligram protein (a).

To analyze gel tissue, 2×2 mm gel samples were fixed for 2 hours in freshly-prepared Karnovsky's buffer (2.5% glutaraldehyde/2% paraformaldehyde in 0.1 M cacodylate buffer) and analyzed as described above.

The PCR program consisted of 94°C for 90 s; 40 cycles of 95°C for 30 s, 53°C for 1 min 30 s, and 72°C for 2 min; followed by final extension at 72°C for 5 min. The amplified fragments were separated on 1% (v/v) agarose gels in TAE buffer, and then photographed and analyzed as described in the REMAP markers section.

The tumor cell fraction was removed, fixed in 1% buffered PFA, and stained and analyzed as above.

Samples were treated with Proteinase K, mixed with agarose gel loading buffer, processed, and analyzed as described above.

TE buffer was analyzed as blank.

The PCR conditions were as follows: 94°C for 2 min; 30 cycles at 95°C for 20 s, 60°C for 40 s, and 72°C for 1 min; and a final extension at 72°C for 5 min. The amplified fragments were separated on the HEGS running system or 2% agarose gels in TBE buffer, and then photographed and analyzed as described above.

Samples of homogenate (20 μl) were mixed with 80 μl of extraction buffer (n=6 7 per group, duplicate samples) and analyzed as described earlier.

For phosphatase experiments, cell lysates were prepared and analyzed as recently described [ 32] in a triton-based lysis buffer with protease inhibitors, but not NaF or Na3VO4.