Sentence examples for buffer and analysed on from inspiring English sources

Finally, cells were washed twice in cold wash buffer and analysed on a Cytomics FC 500 flow cytometer (Beckman Coulter, Wycombe, UK).

After 1 hour at 4°C the pellets were washed in the same buffer, resuspended in SDS PAGE loading buffer and analysed on a 12% PAGE SDS.

Cells were washed in FACS buffer and analysed on FACSCalibur.

Platelets obtained from 12 AMM patients and 12 healthy controls were lysed in 1X Laemelli buffer and analysed on SDS PAGE.

After an additional three washes, beads were resuspended in buffer and analysed on a Luminex 200 running Bio-Plex Software V.5.0 (Bio-Rad, Hercules, California, USA) for determination of PE fluorescence and bead identity.

Reactions were started by the addition of 0.25 mM ATP and reactions were incubated at 30°C for 30 min then stopped by addition of SDS sample buffer and analysed on SDS PAGE followed by Western blotting as above.

Cells were lysed in Laemmli sample buffer (Biorad) and analysed on 12% SDS-PAGE.

The resulting sample was then buffer-exchanged, and analysed on a Q-TOFII (Waters) with the same parameters as described above, except for the acceleration voltage in the collision cell set to 40 V for an optimal visualization of the fragments.

Samples were reconstituted in 10 µl loading buffer before injection, and analysed on a 2 hr gradient for data dependant analysis.

Cells were then washed in staining buffer and analysed by flow cytometry, gating on the CD4+CCR7+ and CD4+CCR7- lymphocyte subsets.

Cells were then washed in phosphate buffered saline (PBS), resuspended and analysed on a Coulter Epics XL flow cytometer.