Sentence examples for buffer and analysed for from inspiring English sources

Blood samples was taken from the tail tip capillary into 10 µl glass capillary tubes and immediately suspended in buffer (500 µl of Biosen analysis buffer) and analysed for glucose on the test day on a Biosen S_Line (EKF-diagnostic, GmbH).

Adherent cells were extracted with RIPA buffer and analysed for ICAM-1 and neutrophil haptoglobin by western blotting.

The beads and protein bound to the fusion protein were washed three times in an excess of homogenization buffer, eluted in Laemmli sample buffer and analysed for bound Cdc42 by western blotting.

After further washing, beads were resuspended in sodium dodecyl sulphate sample buffer and analysed for retention of copurified endogenous 14-3-3 14-3-3 14-3-3 proteins-3 antibody), and for the ability of proteins to bind directly to 14-3-3s in Far-Western overlays.

After incubation, a portion of the buffer was removed, centrifuged and analysed for insulin.

The cells were washed in binding buffer, incubated with a secondary antibody conjugated to fluorescein isothiocyanate (FITC) for 30 min at RT, then resuspended in binding buffer and analysed by flow cytometry (Partec CyFlow Space flow cytometer) and the FlowJo software.

Cells were washed in FACS buffer and analysed on FACSCalibur.

Followed by the addition of 1 mL washing buffer to each assay tube and centrifugation at 200 g for 5 min, the beads were resuspended with 300 μL washing buffer and analysed by a FACSCalibur flow cytometry (BD Biosciences).

Wash buffer (1 mL) was added to each assay tube and centrifuged at 200 × g for 5 min. The bead pellet was resuspended in 300 μL wash buffer and analysed using FACSCalibur cytometer (BD Biosciences, San Jose, CA).

To test the stability of the functionalizing coating, the scaffolds were incubated for 24 h at 37°C in physiologic buffer and then analysed by fluorescence microscopy and FTIR.

Protein of >95% purity (by SDS/PAGE analysis) was exchanged into 50 mM Tris-HCl, 100 mM NaCl (pH 7.5) buffer and concentrated for activity analyses.