Sentence examples for buffer and agitated at from inspiring English sources

Peptides were incubated with equimolar CLR01, CLR03, or buffer and agitated at 1400 rpm at 37°C.

Lyophilized PAP248-286(Ala) was dissolved in PBS (100 µM), incubated with CLR01 (100 µM), CLR03 (100 µM), or buffer and agitated at 1400 rpm at 37°C.

The supernatant was immediately blended with Ni-NTA resin (Novagen), pre-equilibrated with the binding buffer, and agitated at 4 °C for 1 h.

Sensor tips were pre-wet for 15 mins in buffer immediately prior to use, and the microplates were filled with 200 μl per well of diluted samples (VEGF) or buffer and agitated at 1000 rpm.

Sensor tips were pre-wet for 5 min in buffer immediately prior to use, and the microplates used in the Octet were filled with 200 μL of sample or buffer per well and agitated at 1000 g.

The cell pellets were thawed at room temperature for 15 min, lysed in the presence of protease inhibitors in 100 µl lysis buffer I, robotically resuspended in a 96-well block and agitated at 900 rpm for 10 min (5 min in the clockwise direction and 5 min in the counterclockwise direction).

Lyophilized SEM1(45-107) or SEM2(49-107) were dissolved in 0.123 M sodium phosphate buffer to 0.5 mM, passaged through a 0.2 µm filter, and agitated at 37°C and 1400 rpm for 7 days.

A 500 μl volume of a 200 μM aSyn solution in 50 mM TrisHCl, 150 mM NaCl, and 3 mM NaN3 (pH 7.4) were mixed with 500 μl of a 200 μM SERF1a solution in the same buffer, or with 500 μl buffer alone, sealed with Parafilm, taped to the rack of an Eppendorf thermomixer 5436 (Eppendorf), and agitated at 37°C and 1,400 rpm.

(C ) SEM1(45-107) (0.5 mM) was incubated with CLR01 (100 µM) or buffer in the presence or absence of lysine (20 mM) or poly-L-lysine (1 mM) and agitated at 1400 rpm at 37°C for 68 hr.

(B ) PAP248-286 (100 µM) was incubated with CLR01 (100 µM) or buffer in the presence or absence of lysine (20 mM) or poly-L-lysine (1 mM) and agitated at 1400 rpm at 37°C for 68 hr.

(A ) PAP248-286 (1 mM) was incubated with buffer or CLR01 (100 µM) in the presence or absence of lysine (20 mM) or poly-L-lysine (1 mM) and agitated at 1400 rpm at 37°C for 68 hr.