Sentence examples for buffer and NPs were from inspiring English sources

The supernatants were removed and replaced with fresh buffer, and NPs were resuspended by vigorous pipetting.

Definite, indefinite NPs, and 'other' NPs were counted and divided by the total number of NPs produced.

The interactions of buffers and their mixtures with the Au NPs were determined using computational simulations.

To study the protein releasing profile from the UPE NPs, BSA-loaded NPs were resuspended in phosphate buffered saline (PBS) and incubated at 37 °C.

NPs were washed in MES buffer by three wash-spin cycles at 20 000  g.

PEGylated NPs were solubilised in PBS buffer (PBS, 0.5 m EDTA, pH 7.4) such that the final concentration of iron was 1 mg Fe ml 1.

Beads were washed three times with NP-40 buffer and proteins were eluted with Laemmli buffer.

The beads were then washed with NP-40 lysis buffer and complexes were eluted by boiling in Laemmli sample buffer.

Cells were lysed in NP-40 lysis buffer, and lysates were then incubated with either GST or GST fusion protein at 4°C for 2 hours, followed by the addition of 20 µl glutathione-Sepharose 4B beads.

Cells were lysed in NP-40 lysis buffer, and nuclei were resuspended in MNase digestion buffer.

The immunoprecipitated protein was washed three times with NP-40 lysis buffer, and samples were re-suspended in 50  μl of 2 × sodium dodecyl sulfate (SDS) sample buffer, incubated at room temperature for 10 min and then assayed by SDS-10SDS-10%crylamidelectrophoresisorelectrophoresis