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The functionality of the tool for root cause investigation was successfully demonstrated in a model protein study with lysozyme, cytochrome c, and ribonuclease A. Deviating chromatograms were generated by deliberately caused errors in the process parameters flow rate and sodium-ion concentration in loading and elution buffer according to a design of experiments.
Packets arrive from the higher layers into the transmit buffer according to a certain random process (e.g., a Poisson or Bernoulli distribution).
Whole cell extracts were prepared using the Nonidet P-40 lysis buffer according to a previously described method (31).
DNA was isolated from the tissues incubated with the lysis buffer according to a standard protocol [ 13].
Apparent equilibrium constants of dissociation (KD) for triangles were determined in TMS buffer according to a previously described procedure.
In the way the technique is typically applied, the peptides are released from the chromatography column by the gradual addition of an organic buffer according to a linear function.
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The latter solution was desalted using a PD-10 desalting column (GE Healthcare) and TDG buffer according to the manufacturer's instructions.
Total proteins from H9c2 cells were extracted using radio-immunoprecipitation (RIPA) buffer according to the manufacturer's protocol (Santa Cruz Biotechnology, CA).
For electrophoresis, 4 12% precast NuPage® Bis-Tris gradient gels were resolved using Mops buffer according to the manufacturer's instructions (Invitrogen).
One gram of tissue was extracted with 10 ml of extraction buffer according to an established method [ 68].
Tissue samples were fixed in 10% neutral formalin, buffered according to Lillie and embedded in paraffin.
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