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The latter solution was desalted using a PD-10 desalting column (GE Healthcare) and TDG buffer according to the manufacturer's instructions.
Total proteins from H9c2 cells were extracted using radio-immunoprecipitation (RIPA) buffer according to the manufacturer's protocol (Santa Cruz Biotechnology, CA).
For electrophoresis, 4 12% precast NuPage® Bis-Tris gradient gels were resolved using Mops buffer according to the manufacturer's instructions (Invitrogen).
Sections were preheated in Dako EnVision FLEX + Target Retrieval Solution, High pH, and rinsed in Dako wash buffer according to the manufacturer's instructions.
It then allocates the decoder to an input buffer according to some scheduling scheme.
Total protein was extracted using radioimmune precipitation buffer protein lysis buffer according to protocols (Beyotime, Shanghai, China).
In Figure 1, the classifier classifies the mixed traffic at Layer 2 data buffer according to the type of traffic.
After incubation, the samples were treated with NuPAGE® LDS sample buffer according to the manufacturer's instructions and finally 0.05 nmol of sample loaded on the gel.
Packets arrive from the higher layers into the transmit buffer according to a certain random process (e.g., a Poisson or Bernoulli distribution).
After incubation, the samples were treated with NuPAGE® LDS Sample Buffer according to the manufacturer's instructions and loaded on the gel.
The playout buffer is emptied at the nominal sequence frame rate, and the frames are extracted from the buffer according to their decoding order.
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