Sentence examples for buffer Qiagen to remove from inspiring English sources

Following treatment with the gDNA wipeout buffer (Qiagen) to remove potentially contaminating genomic DNA, RNA quality was assessed by agarose gel electrophoresis and its quantity determined using a Nanodrop spectrophotometer (Nanodrop Technologies/Saven Werner, Norway).

For mouse bone marrow-derived macrophages (BMDMs), bone marrow cells from female C57BL/6J mice at 8 weeks of age were treated with Buffer EL (Qiagen) to remove red blood cells, and cultured for more than 7 days with DMEM containing 10% FCS supplemented with 5% supernatant of CMG14-12 cells producing mouse M-CSF (Takeshita et al., 2000).

RNA clean-up was carried out again with an RNeasy Mini Kit (Qiagen) to remove any contamination introduced by TURBO DNase buffers.

The sections were incubated and Binding buffer was then added to the lysate and transferred to a gDNA Eliminator spin column (Qiagen) to remove genomic DNA.

RNA was treated on-column with RNase-free DNase (QIAGEN) to remove contaminating genomic DNA.

The remaining mRNA was purified (RNeasy; Qiagen) to remove excess MgCl2prior to ribosome display.

The probes were purified using QIAquick spin columns (Qiagen) to remove unincorporated nucleotides.

All RNAs were treated with DNase I (Qiagen) to remove genomic DNA.

The RNA was then treated with DNaseI (Qiagen) to remove any contaminating genomic DNA.

Samples were digested with DNase I (Qiagen) to remove contaminating genomic DNA.

Samples were treated with DNase (gDNA wipeout buffer, Qiagen) to eliminate any potential DNA contamination.