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Protein extraction buffer (Buffer) served as a negative control.
This critical limit is independent of pH, electrolyte, ionic strength, buffer, buffer capacity or surface roughness.
The refolded S228P was successfully obtained by one-step dilution of the 6 mol/L GdnHCl denatured proteins in the refolding buffer (buffer A).
Solubility of feed N in borate phosphate buffer (buffer soluble N; BSN) is widely used in modern feed evaluation systems as a cornerstone for calculation of rumen N degradability.
Initially, ZIKV helicase172 617 (storage buffer: buffer D) at 5 mg/mL was incubated with ssRNA (GenePharma) at 0.2 mmol/L (~2-fold molar excess) at 18°C for 1 h.
mda-7/IL-24 IBs were washed using '3 + 1' washing buffers (about 1 g IBs were solubilized in 30 mL washing buffer): buffer I, buffer II, buffer III, and deionized water (Table 2).
Table 2 The components of '3 + 1' washing buffers Washing buffer Urea (M) EDTA (mM) Tris (mM) NaCl (mM) Triton X-100 pH of the buffer Buffer I 2 10 20 100 1 8.5 Buffer II 4 10 20 0 2 8.5 Buffer III 2 10 20 0 1 9.5 Deionized water 0 0 0 0 0 6.5 to 7.0.
PCR was conducted in a 25 µL reaction with 7.0 µL buffer (buffer 5.0 µL + dNTPs mixture 2.0 µL), 0.50 µL each of forward and reverse primers, 0.25 µL PrimeSTAR HS DNA polymerase (Takara Bio Inc., Shiga, Japan), and 0.50 µL template DNA.
Protein extracts were obtained using Cell Extraction Buffer buffer (BioSource International, CA, USA) plus protease inhibitor cocktail (Sigma).
Control dialysis membranes contained either buffer, buffer contaminated with the bacteria on which the D. caveatum amoebae grow, or D. discoideum at 107 cells/mL.
