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The dissolved RNA was treated with DNAse I buffer (Ambion) to remove gDNA contaminations, prior to centrifugation and collection of the aqueous phase.
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Purified mRNA was eluted in 25 µl of TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) and was further purified with the MEGAclear™ kit (Ambion) to remove small RNAs and small contaminants.
Total RNA was isolated from the callus (approximately 100 mg) by the TRIzol reagent method (Invitrogen) and treated with TURBO DNA-free™ DNAmbionmbion) to remove residual DNA.
Contaminating chromosomal DNA was removed by TurboDNase (Ambion/Applied Biosystems) and samples were treated to MICROBEnrich™ (Ambion) to remove eukaryotic RNA.
All RNA samples were treated with DNase (DNA-free Kit, Ambion) to remove contaminating DNA.
The T7 transcripts were purified on NucAway Spin columns from Ambion (to remove unincorporated nucleotides).
Extracted RNA was treated with DNAse I (Ambion) to remove traces of genomic DNA.
Total RNA was treated with DNase I (Ambion) to remove contaminated genomic DNA.
RNA samples were treated with Turbo DNA free (Ambion) to remove genomic DNA contamination.
The total RNA was treated with RNase-free DNase I (Ambion) to remove any DNA contamination.
Total RNA was treated with Deoxyribonuclease I (Ambion) to remove contaminating DNA.
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