Sentence examples for budding reaction from inspiring English sources

Cryo-electron tomograms of a reconstituted COPII budding reaction.

20% of the COS7 cells prior to the budding reaction serves as a loading control (20% donor).

It is common to all models that ESCRT-III subunits are not consumed in the budding reaction, since they are not localized inside the buds but remain at bud necks.

The parasite protein, SAR1 (PF3D7_0416800), is a small GTP-binding protein of 192 amino acid residues, which is involved in the crucial step of budding reaction in vesicle-mediated secretory pathway.

We hypothesize that these results reflect the loss of one or more key components during cytosol preparation from mouse tissues, or the presence in these preparations of an inhibitor to the budding reaction.

In agreement, the budding reaction has been reconstituted in vitro and used to demonstrate that Vps20, Vps32 and Vps24 suffice to form multivesicular bodies, whereas neither Vps4 itself nor Vps2, the major recruiter of Vps4 (Obita et al., 2007), are required for one round of vesicle budding, although they are required for initiating new rounds of vesicle formation (Wollert et al., 2009).

Briefly, after budding reactions were performed as described above, the samples were diluted with an equal volume of buffer that contained 500 mM KCl to release COPI vesicles.

Like all vesicle budding reactions, the formation of intralumenal MVB vesicles requires three distinct steps.

In vitro budding reactions release vesicles containing Pex3, dependent on Pex19, cytosol and ATP [ 39,40].

In Fig. 6D, microsomal membranes form THY9-1 (KTR4-3HA:: HIS3 SVP26), MIY259 (KTR4-3HA:: HIS3 Δ erv41) and MIY258 (KTR4-3HA:: HIS3 Δ erv46) were used for the budding reactions.

First, we have repeated our in vitro budding reactions with a mutant form of Arf1 that impairs dimerization (Arf1Y35A; see Beck et al., JCB 2011).