Sentence examples for budding from the plasma from inspiring English sources
Based on a study of 'broken' synaptosomes it was proposed that such a conversion occurs via the same clathrin-mediated budding reaction that drives clathrin-mediated budding from the plasma membrane (Takei et al., 1996).
The release of shedding vesicles is a result of direct budding from the plasma membrane, while exosomes are produced by exocytosis of multivesicular bodies (Zomer et al., 2010).
However, here we demonstrate that HIV Gag and murine leukemia virus (MLV) Gag also drive assembly intracellularly in cell types including 293 and HeLa cells, previously believed to exclusively support budding from the plasma membrane.
No virus was detected budding from the plasma membrane in mouse T cells (Figure 9, lower panels), but some particles were observed budding into the nuclear membrane.
The involvement of the spectrin cytoskeleton during CME has been examined by others and shown to be excluded from clathrin-coated pits to encourage budding from the plasma membrane [23], [35], [36].
This autoprocessing is usually delayed until GagProPol and Gag polyproteins assemble at the cell membrane and is followed by further cleavage of Gag and other GagProPol polyproteins during and/or just after budding from the plasma membrane [2], [3].
Since L. mexicana exosomes bud from the plasma membrane and their surface contains GPI-anchored gp63, interaction of the exosomes with the macrophage plasma membrane and its fusion could be another route for entrance of gp63 into the host macrophage.
However, other viruses, such as HIV, were reported to occasionally bud from internal multi-vesicular bodies [ 45], even though they usually bud from the plasma membrane.
It is recruited to membranes during endocytosis and forms basket-like structures around vesicles as they bud from the plasma membrane.
Transferrin-positive puncta that do not contain FM1-43FX are likely to represent clathrin-coated pits that have not budded from the plasma membrane.
This is a significant improvement in time resolution, and hence in our confidence that the detected vesicles have just budded from the plasma membrane (Sabharanjak et al., 2002; Kirkham et al., 2005; Glebov et al., 2006).
