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Gene and 3′ UTR conservation score were downloaded from UCSC genome browser websites.
The datasets for poplar could be obtained from PlexDB and eFP browser websites while that for grapevine and castor bean from PlexDB and a RNA-sequencing project, respectively.
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Sequence alignments of GABRB2 gene among different species were downloaded from UCSC Genome Browser website.
Genomic positions of resulting sequences were determined on the rat genome (rn3) by BLAT search performed at the UCSC genome browser website (http://genome.ucsc.edu).ucsc.edu
The bovine genome (version 4.0, released October 2007) sequences for all chromosomes and unmapped scaffolds were downloaded from the UCSC genome browser website (http://genome.ucsc.edu).ucsc.edu
To explore evolutionary conservation across different species, we measured for each polymorphic position the conservation score of the Genome Browser website (assembly March 2006, http://genome.ucsc.edu/), based on multiple alignment of 17 vertebrate species [34].
The other four resources, such as the human reference genomic sequences (hg18), the mRNA/EST sequences, the 'gbCdnaInfo.txt' flat file (alignment between the human mRNAs/ESTs and genome sequences), and the 'tissue.txt' flat file (human mRNA/EST library information) were all downloaded from the UCSC genome browser website [42].
According to the alignment information downloaded from the UCSC genome browser website, all of the expressed sequences overlapping the same RNA editing site were grouped together and classified into two groups, edited or unedited, based on whether the nucleotide at the editing position is a guanosine (G) or adenine (A).
We downloaded the hg18 gene annotations from the UCSC genome browser website [ 42].
Nucleotide conservation scores were downloaded from UCSC Genome Browser website [ 35].
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