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A hepatocyte with dark brown cytoplasm and nucleus was considered positive cells [24].
Caspase-3-positive cells showed brown cytoplasm, while the cytoplasm of caspase-3-negative cells was blue (Figure 6). Figure 4 Numbers of caspase-3-positive cells and PCNA positive nuclei (counting area = 3,500 μm 2 ).
Brown, cytoplasm.
The JNK3 positive cells appeared brown cytoplasm and measured by UV spectrophotometer (Beckmann USA) in four non-overlapping visual fields in each section.
Tumour cells with brown cytoplasm were considered positive and staining intensity was classified as: weak brown (1+); moderate brown (2+) and strong brown (3+).
The averaged intensity score of cellular VEGF for the MSeA-treated tumor was 1.0 (weak staining), while as the same score for the non-MSeA treated control tumor was greater than 2.0 (dark brown cytoplasm staining).
Similar(53)
The cells were visualized using a microscope (Olympus) and the immunoreactivity was identified as brown cytoplasmic staining in cytoplasm.
For each tumor, more than 250 cells were counted for active caspase 3 positivity (brown cellular cytoplasm) and the percentage calculated.
The immunoexpression of s100, bcl2 end MPO (Group 2) have been confirmed by the presence of brown stained cytoplasm in cell infiltration.
We took the brown-colored cytoplasm cells as positive expressions.
The mean staining intensity was estimated and expressed as weak (yellow or light-brown cytoplasmic or nuclear staining), moderate (dark-yellow or medium-brown staining, or a mixture of light and dark staining) or strong (dark-brown staining in nucleus or cytoplasm).
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