Sentence examples for broth which was then from inspiring English sources

For two-stage cultivation, 3% (v/v) of the inoculum from transformant C. necator culture was transferred into fresh NR broth which was then incubated for additional 24 h.

The supernatant with unattached phages was removed, and the pellet re-suspended in 1 ml of ice cold LB broth, which was then added to 9 mls of LB already shaking at the desired experimental temperature.

Human fecal samples were suspended and poultry samples and carcasses were massaged in nutrient broth, which was then incubated overnight at 37°C and stored as aliquots at –80°C in glycerol (14 ).

One colony from the agar plate was used to inoculate 10 mL MRS broth which was then incubated at 37°C for 24 h, prior to any experimental assay.

Alternatively, C. albicans was inoculated into soft (0.5%) Lysogeny Broth agar, which was then was used to overlay the plate containing the actinomycete.

After overnight incubation at 37°C, a sterile cotton swab was used to transfer the entire growth from each plate into Luria broth containing 30% (vol/vol) glycerol, which was then frozen at –70°C until required.

The crude extract was dissolved in 50% DMSO at a concentration of 50 mg/ml which was then diluted with nutrient broth to 1 mg/ml.

Solvent extraction from alkalized broth and two-step distillation provided highly pure diaminopentane (99.8%), which was then directly accessible for poly-condensation.

Pre-warmed LB broth (400 μL at 37°C) was added to the transformation reaction which was then maintained at 37°C for 90 minutes in the shaker incubator.

Broth samples (1 mL) were collected and centrifuged at 4 °C and 12,000g for 2 min to obtain the supernatant, which was then diluted and assayed.

Single black colonies were selected from the XLDA plates and used to inoculate five 10-ml tubes of BHI broth (Becton Dickinson), which were then incubated at 37 °C for 24 h.