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Stock cultures grown in brain heart infusion (BHI, Becton Dickinson, France) broth were stored at −80°C in 25% (v/v) glycerol.
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The fermentation was terminated after 5.5 days and the broth was stored at 4°C until processed.
A 1 mL aliquot of the cultured LIM broth was stored at -80°C for subsequent Group B Streptococcal PCR.
Listeria monocytogenes wild type (10403S) stock was stored at –80° in brain and heart infusion (BHI) broth containing 25% glycerol.
Stock cultures were stored at -80°C in LB broth containing 30% glycerol.
The culture broth and the mycelium were stored at −80°C until further research.
Selected colonies (576-768) from each library were inoculated into 150 μl aliquots of LB kanamycin broth and duplicate glycerol stocks were stored at -80°C.
For long-term storage, samples were suspended in YEGC broth containing 10% (v/ v) glycerol and were stored at −80 °C.
From these a single colony was cultured over night in Sabouraud broth, and 25% glycerol stocks made; these were stored at −80°C.
The microorganisms were stored at −70°C in Sabouraud Dextrose Broth (SDB, Merck® - C. albicans) and Mueller-Hinton Broth (Difco® - bacteria) with 15% glycerol.
All gram-positive and catalase-negative isolates were stored at −80 °C in MRS broth containing 15% glycerol (v/v).
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