Each sample was processed by the antibiotic broth elution assay [10].
A 70 L fermentation of a H. rickii strain was processed by separating the mycelia from the culture broth and extraction of the corresponding biomass with acetone.
In our microbiology lab, CVC tips are processed by first adding 2 ml of D/E Neutralizing Broth (Neogen, Lansing, MI, USA) to neutralize any residual disinfectants or antimicrobials.
The authors recommend that these samples are processed by inoculation on to agar plates (primary culture) as well as an enrichment broth for extended culture.
In general, soil to be tested for the presence of SRE can be processed by suspending in sterile water or buffer and shaking for a couple of hours, or better still, first enriched by incubating in a selective broth (pectate enrichment broth – PEB; see below) (Pérombelon & van der Wolf, 2002) before dilution and plating onto agar selective media (see below).
Early morning samples were processed by the modified Petroff's method [ 22], stained by Ziehl-Neelsen Carbol Fuchsin, microscopically examined, and cultured on Lowenstein-Jensen (LJ) slopes (Himedia, India) and in Dubos broth (Himedia, India).
The cell-free broth supernatants were processed through C18 SPE mini columns and analysed accordingly by HPLC for the presence of TBP.
For evaluating the strength of interaction, β-galactosidase activity was measured with cells grown in LB broth and samples were processed as recommended by the supplier.
Stool samples were processed as for isolation of ESCREC, but after the initial growth the MacC-broth-CTX was added to selenite broth.
Because food orders are processed 24 hours ahead, patients who may have been forced to order from the liquids-only menu can be cleared for solid foods by their doctors one day but still face another day of broth.
