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The pH of the broth was maintained at 5.5 by adding 6.5 N sodium hydroxide.
The pH of the culture broth was maintained at pH 7.0 by feeding in the VFA mixture (500 g/L) with C/N ratio mentioned earlier.
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Bacteria were routinely cultured in Luria broth (LB) at 37°C.
E. coli was maintained at 250 mL of Luria Bertani (LB) broth at 37 °C, with shaking at 200 rpm for 12 h until the OD600 value was approximately 0.6.
The dissolved CO2 in the culture broth could be maintained at a very low concentration; the maximum value (~17 mg/L) was one-fifth that of the control (Fig. 2b).
The stock cultures were maintained at −85 °C in M17 broth supplemented with 20% (v/v) glycerol.
Stock cultures were maintained at −70 °C in a suspension of tryptic soy broth (TSB) containing 15%% glycerol.
Gram-positive and Gram-negative bacteria were maintained at 37 °C in tryptic soy broth (TSB) and Luria-Bertani broth (both obtained from Sigma), respectively.
Isolates were maintained at -80°C in YM broth containing 20% (v/v) glycerol.
These strains were maintained at −80°C in Brucella broth (Becton Dickinson, Franklin Lakes, NJ, USA) containing 25% (vol/vol) glycerol.
The strains were maintained at −20°C in MRS broth (Microbiol, Cagliari, Italy) with 15% (v/v) glycerol and propagated three times in MRS broth for activation prior to experimental use.
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