Sentence examples for broth was extracted using from inspiring English sources

Genomic DNA from an overnight culture of P. aeruginosa PAO1 grown in LB broth was extracted using Qiagen (Valencia, CA) 500/G Genomic-tips and Genomic DNA Buffer Set following the protocol of the supplier with the exception of increasing the amount of wash buffer used to 3x the recommended volume.

Single colonies from the endpoints of MA lines were isolated, grown in Lysogeny Broth, and genomic DNA was extracted using a Qiagen DNeasy Blood & Tissue Kit.

The bacteria were cultivated in LB broth at 28°C for 24 hours and the chromosomal DNA was extracted using conventional SDS lysis and phenol-chloroform extraction method.

All the strains were cultivated in the Luria-Bertani broth and the genomic DNAs were extracted using the conventional phenol-chloroform extraction method.

Surface-lying mycelial mats were moved aside with sterile pipette tips, and broth was extracted for use in pre-screening for cellulase expression by dot blot analysis.

4 ml of culture broth was extracted with an equal volume of acetone and residual acetone was removed by evaporation.

The broth was extracted with EtOAc (3 × 50 mL) and concentrated under reduced pressure to obtain a brown gum (broth extract, 34 mg).

Culture broth was extracted with dichloromethane and mycelia was freeze-dried, pulverized and extracted with dichloromethane.

Filtered culture broths were extracted with an equal volume of ethyl acetate or dichloromethane using a separatory funnel.

The fermentation broths were extracted with acetone hexane.

Aeromonas species and subspecies were grown in tryptic soy broth and DNA was extracted for use as the template in standard PCR reactions.