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For the broth microdilution test, 50 μL of bacterial suspension in exponential phase of the growth was added to the wells of a sterile 96-well microtiter plate already containing 50 μL of twofold serially diluted propolis extracts in growth medium.
Antileptospiral assay were carried out using broth microdilution test [ 38, 39].
Susceptibility testing was done using an automated broth microdilution test (Vitek; bioMérieux, Durham, NC, USA).
The results of broth microdilution test demonstrated that the crude extract and purified xanthones from mangosteen possessed antileptospiral activities.
The minimum inhibitory concentration for the 11 L. plantarum strains was determined by a broth microdilution test.
In vitro susceptibility test was performed by broth microdilution test as described by the National Committee for Clinical Laboratory Standards (NCCLS) guidelines [ 4].
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The isolates were referred to a central monitor for reference NCCLS broth microdilution testing, identification confirmation and patient demographic analysis.
Preparation of inocula for the broth microdilution testing was performed in accordance with CLSI documents M27-A2 [19] with RPMI 1640 medium.
Sensititre™ panels (Trek Diagnostic systems, UK) were used for broth microdilution tests, and antibiotic discs (Abtek Biologicals Ltd ,Liverpool, UK) for disc-diffusion tests.
Broth microdilution testing of clinical isolates revealed 88.9% susceptibility to SXT and 44.4% to levofloxacin.
Broth microdilution testing was performed in accordance with Clinical and Laboratory Standards Institute document M27-A3 guidelines [ 13].
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